Before the release of ImageJ in 1997, a similar freeware image analysis program known as NIH Image had been developed in Object Pascal for Macintosh computers running pre-OS X operating systems.Fiji uses modern software engineering practices to combine powerful software libraries with a broad range of scripting languages to enable rapid prototyping of image-processing algorithms.
CELLPROFILER uses a customized pipeline, including input and analysis modules, to process images. For comparison purpose, we used a naïve setup and choice of parameters. If necessary you can discover ImageJ in the same If this is not the case you might still nd parts of the workshop interesting. Ideally you have already used ImageJ before but you know nothing or very little about programming.